Invitrogen Qubit 4 - Reading Samples; Preparation and Reading Procedures

24 Qubit
™
4 Fluorometer User Guide
Read samples
Before you begin
•
Calibrate the Qubit
™
4 Fluorometer as described on page 18. (Run the
appropriate standards or accept the values from the previous calibration.)
•
Prepare the samples. Refer to the instructions provided with the assay.
Note: Incubate the samples for the appropriate amount of time after
mixing them with the working solution (2 minutes for the Qubit
™
DNA
and RNA assays, 15 minutes for the Qubit
™
protein assay).
Read samples
1. In the Read standard (for Qubit
™
quantitation assays) or Ready for samples
screen (for Qubit
™
RNA IQ assay), press Run samples.
2. In the Sample volume screen, select the sample volume and units for the Qubit
™
quantitation assays or the Qubit
™
RNA IQ assay:
a. Press the + or – buttons on the wheel to select the sample volume added
to the assay tube (between 1 and 20 µL).
b. For the Qubit
™
quantitation assays, select the units for the output sample
concentration from the dropdown menu.

Note: In the RNA IQ assay, there are no units associated with the RNA IQ
value (i.e., RNA IQ#) and only sample volume is required.