Invitrogen Qubit 4 - Default Chapter; Table of Contents

Qubit
™
4 Fluorometer User Guide 1
Contents
About this guide ............................................................................................................ 3
1. Product information ................................................................................................ 5
Product contents .............................................................................................................................................. 5
Product description ......................................................................................................................................... 6
Instrument exterior components ................................................................................................................... 7
Product specifications ..................................................................................................................................... 8
2. Getting started ........................................................................................................ 9
Set up the Qubit
™
4 Fluorometer ................................................................................................................... 9
Optional: Configure instrument settings ..................................................................................................... 11
Guidelines for using the Qubit
™
4 Fluorometer ........................................................................................ 16
3. Perform assays ..................................................................................................... 17
Overview ........................................................................................................................................................ 17
Optional: Use Reagent Calculator to prepare Qubit
™
Working Solution ............................................... 18
Calibrate assays on the Qubit
™
4 Fluorometer .......................................................................................... 20
Read samples ................................................................................................................................................. 24
Results ............................................................................................................................................................. 26
Fluorometer mode ......................................................................................................................................... 29
Ion Sphere
™
Assay ......................................................................................................................................... 31
4. Manage data .......................................................................................................... 32
Introduction ................................................................................................................................................... 32
View detailed sample data ........................................................................................................................... 32
Rename data files........................................................................................................................................... 35
Export data ..................................................................................................................................................... 36
Delete data ...................................................................................................................................................... 39
5. Manage, import, and create assays ....................................................................... 40
Overview ........................................................................................................................................................ 40
Manage assays ............................................................................................................................................... 40
Show/Hide assays ........................................................................................................................................ 41
Move assays ................................................................................................................................................... 43
Import assays ................................................................................................................................................. 45
Create your own assay for the Qubit
™
4 Fluorometer .............................................................................. 50
6. Instrument maintenance ....................................................................................... 51
Maintenance and cleaning............................................................................................................................ 51
Software updates ........................................................................................................................................... 52
Device verification test ................................................................................................................................. 54