Invitrogen Qubit 4 - Sample Quality and Measurement Errors; Quality Results and Error Messages

28 Qubit
™
4 Fluorometer User Guide
Results screen –
Sample quality
The Results screen displays the results of the sample run.
• If the results are within the assay’s range, the sample quality values are
displayed. The result presented in the circle (i.e., RNA IQ#) is the total value
for the RNA sample integrity and quality, which is the calculated percentage
of large and small RNA in the sample.
The RNA IQ# is based on a scale of 1–10, similar to other RNA quality scores.
A small RNA IQ# in the assay
indicates that the sample is comprised of mainly
small RNA and a larger RNA IQ# indicates that the sample consists of mainly
large RNA or structured RNA (mRNA, tRNA, rRNA).
The actual percentages of small or degraded RNA and large or intact RNA in
the sample are displayed below the RNA IQ#.
Measurement error
If the results are outside of the assay’s range, a “Measurement error” message is
displayed.
Measurement error – Sample
concentration too low
Measurement error – Sample
concentration too high