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Qubit
™
4 Fluorometer User Guide
• The sample is out of range. Use a sample that is more concentrated or use a
lower dilution (for example, 20 µL in 180 µL instead of 10 µL in 190 µL).
• For Qubit
™
quantitation assays, view the
Fluorescence vs. Concentration graph in the
Results screen to confirm that the values for
the samples fall between the values of the
standards (page 27).
• Ensure that you have prepared the Qubit
™
working solution correctly (1:200 dilution
using the buffer provided in the kit).
• Ensure that you have prepared the standard
tubes correctly (10 µL of each standard in
190 µL of Qubit
™
working solution).
• Ensure that the standard and sample tubes
are filled to 200 µL.
• Protect the Qubit
™
reagent and working
solutions from light.
• Select the correct Qubit
™
4 Fluorometer assay for the Qubit
™
assay you are
performing and calibrate the fluorometer correctly. Standards must be used
in the correct order.
•
Ensure that the assay is performed entirely at room temperature.
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