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11. Technical Description
11. Technical Description
Due to basic principles of physics and the physi-
ology of the eye, all methods – not just those in
microscopy – have performance limits. There-
fore, observe the following information for cor-
rect use of the microscope.
Performance data of the objectives
The Leica DMi1 microscope is based on the tube
length ∞ (infinity) and a tube lens focal length of
f = 200 mm.
!
Caution:
Therefore, only objectives with the engraving ∞
and the thread dimension M 25 may be used.
Objective marking
Examples and meaning of the symbols:
∞ / –
HI PLAN 10x/0.22
∞ / 0.17
N PLAN 40x/0.65
∞ / 0 / D
N PLAN 50x/0.75
∞ Objective for infinite tube length (∞).
— The objective can be used with and
without cover slip.
0.17 The objective may be used with a cover
slip of the standard thickness 0.17 mm
only. If the cover slip is missing or the
thickness of the cover slip deviates
greatly, significantly decreased perfor-
mance will result, particularly for high
objective apertures (see below).
0 Application without cover slip, e.g. for
cell smears, incident light. Not suited
for inverted microscopes.
D or
(A, B, C) Eyepoint of the objective (important for
Modulation Contrast IMC, for example)
L Long working distance.
10x/0.22 Magnification and aperture. The aper-
ture determines the resolution, focus
depth, contrast and brightness of the
microscope image. Objectives with
built-in iris diaphragm have the maxi-
mum and minimum aperture engraved,
e.g. 0.85-0.55.
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