26
Transmitted-light stand, bright
and
dark field
The illumin
at
i
on
of
the transmitted-light stand bright
and
dark
fie ld consists of a fi
be
r-optic light guide and a light source.
.1.\.
Observe the safety regulations from the
~
manufacturer of the
light
source.
..,.
Unplug the fiber-optic light guide f
rom
t
he
con11ect
i
on
in
the stand .
..,.
Fit the side-faced column to the transmitted-light base
as
described
on
page
23
.
..,.
Fit the microscope carrier and stereomicroscope
as
described
on
page
34
.
..,.
Attach t
he
fiber-optic light guide at t
he
stand
and
t
he
light source.
Starting
up
Before
us
i
ng
the light source, read the corresponding use r
manual.
Transmitted light.
bright
field
Transmitted light,
dark
field
Bright field is suitable for examining translucent objects featur-
ing contrasting structures. The object
is
di
r
ec
tly illuminated
from
be
l
ow
and
is
see
n in its natural colors against a bright
background.
..,.
Slide handle to the right until the
stop: bright
field.
Dark field offers
mo
re information for objects poor
in
contrast
with rather
poorly marked or very fine structures.
In
this case,
the light beams penetrate the object at a flat angle.
Fine
detail
structures
and
contours contrast brilliantly
and
brightly from
dark background.
..,.
Slide the handle out to the right:
dark
fiel
d.
Leica M Stereomicroscopes - Assembly
Transmitted-light stand
HL
Column-+ Base
The
column seat contains Alien screws.
..,.
Remove the glass stage plate .
..,.
Invert the transmitted-light base
and
place it
on
a table .
..,.
Hold column against the column seat from underneath.
..,.
Insert the Alien screws into the
co
l
umn
from the top
and
tighten them .
..,.
Invert the transmitted-light base
and
insert the glass
stage plate .
..,.
Fit
additional components such
as
the microscope carrier
and optics carrier (page
34)
.
Illumination Add a very luminous cold light source
and
a light guide with
Accessory condenser
-+Basis
Use
Tilted mirror
a
channel to the transmitted-light stand
(TVC,
active 0 9
mm).
..,.
Insert light guide into the base at the rear.
..,.
Connect light guide to the light source.
For
stereomicroscopes with hig h apertures such
as
Leica
MZ12s,
MZ16
and
MZ16
A
wit
h objective
1.6
x, the resoluti
on
can
be
increased
by
inserting
an
accessory condenser.
..,.
Push the accessory condenser
in
to the l
ens
holder
at
the
underside of the transmitted-light base
and
secure it
with
2 Alien screws.
..,.
Turn
on
the light source according to the manufacturer's
user
manual
and
adjust the light intensity .
..,.
Place a transparent object
on
the glass stage plate
and
focus.
The
built-in mirror features a clear
and
an
obscured side and
can
be
rotated
and
moved.
The
obscured side provides a dif-
fused illumination. The angle of
in
cidence
in
the specimen
plane changes depending on the position of the mirror
so
that
the transmitted light bright field
via
oblique illumination can also
generate
an
illuminati
on
similar to dark field.
..,.
Rotate
and
move the mirror with t
he
black rota
ry
kn
ob on
the
right si
de
.
..,.
Adjust exact bright field
and
maximum illumination: move
mi
rror to the stand column until it stops.
Rotate the mirror
until it engages in the
45
° position.
Le
ica
1\1
Ster
eo
microscopes - As
se
mbly
27
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