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Nikon A1+ Standard Operating Procedure

Nikon A1+
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Incubator
on/off
Oko-lab stage incubator
(11)
C0
2
tube for
incubator
Using the stage top incubator
1. Please ask Sebastian the first time
you want to use the stage top
incubator/holder for more in-
depth instructions
2. Open C0
2
valve on tube
3. Turn on incubator
4. Change to the incubator sample
holder (see previous page for
instructions)
5. Wait until the temperature and C0
2
levels reach the desired values (see
display).
*If you want to use the 60x objective with the
objective heater you need to connect the loose
connector from the objective with the
matching connector from the incubator
controller.
1
2
3
4

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Nikon A1+ Specifications

General IconGeneral
TypeConfocal Microscope
SoftwareNIS-Elements
Laser Lines405 nm, 488 nm, 561 nm, 640 nm
DetectorGaAsP PMT detectors
Scanning MethodGalvanometer-based scanning
Objective TurretMotorized
DimensionsVaries by configuration
WeightVaries by configuration
Scan SpeedUp to 30 fps (512 x 512 pixels)
Objective LensesCompatible with Nikon CFI series objectives
Frame Rate30 frames per second

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