Nikon A1+ Standard Operating Procedure

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Finding your sample
1. Put your sample in the appropriate holder, if you have slides make
sure the coverslip is facing down.
2. Start with either 10x or 20x objective
3. Navigate to a suitable place in your sample with the joystick.
4. Press “Eye port” to change the light path to the eye piece, if
needed press the Sola button (on Sola pad) to activate the LED
lights.
5. Use the coarse focusing mode and find focus (for slides around Z=
2100-2200, for 30mm petri dishes around Z=2500-2700)
6. Press “Eye port” again to switch to confocal mode (the first time
the system is started do this twice, this is a bug in the software).
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Nikon A1+ Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Scanning Method	Galvanometer-based scanning
Objective Turret	Motorized
Dimensions	Varies by configuration
Weight	Varies by configuration
Scan Speed	Up to 30 fps (512 x 512 pixels)
Objective Lenses	Compatible with Nikon CFI series objectives
Frame Rate	30 frames per second