Nikon A1+ Standard Operating Procedure

Motorized stage
power
Brightfield lamp power
A1 controller
Laser
on/off
key
Laser
buttons
(2)
Turning on/off the system
1. Turn on computer
2. Power on A1 controller and laser
power supply.
3. Turn the key and select the
needed lasers by pressing the
buttons.
4. Turn on stage power supply and
DIA lamp if bright field is needed
5. Power on microscope, wait until
startup sequence is finished.
6. Log in with your LBIC account
and start NIS-Elements AR
7. Turn off system in reverse order
4

Nikon A1+ Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Scanning Method	Galvanometer-based scanning
Objective Turret	Motorized
Dimensions	Varies by configuration
Weight	Varies by configuration
Scan Speed	Up to 30 fps (512 x 512 pixels)
Objective Lenses	Compatible with Nikon CFI series objectives
Frame Rate	30 frames per second