Nikon A1+ Standard Operating Procedure

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Confocal Acquisition: Quick Start
1. Setup your channel requirements
2. HV (Gain), start at 100 for 405/640 and 25 for 488/561 PMTs
3. Offset adjustment, leave at 0
4. Laser Power, start at 2%
5. Start with a scan size of 512x512 and scan speed of 2.2 µsec.
6. Select “Normal” and “Ch Series” if using more than one laser
7. Select 1.2 AU for Pinhole
8. Start “SCAN”
9. Adjust detector and lasers settings if needed
10. Change pixel size by manually changing zoom factor in A1+ Scan Area” dialog
(View>Acquisition Controls>A1 Scan Area) or pressing Nyquist for optimal pixel
size for the selected objective and pinhole size.
11. Quickly Capture an image with the current settings. Image must be saved
manually afterwards. Save as .nd2.
*If you need to go back to the eyepieces to check your slides, click “Eye Port”,
click it again to return to the confocal!
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Nikon A1+ Specifications

General

Type	Confocal Microscope
Software	NIS-Elements
Laser Lines	405 nm, 488 nm, 561 nm, 640 nm
Detector	GaAsP PMT detectors
Scanning Method	Galvanometer-based scanning
Objective Turret	Motorized
Dimensions	Varies by configuration
Weight	Varies by configuration
Scan Speed	Up to 30 fps (512 x 512 pixels)
Objective Lenses	Compatible with Nikon CFI series objectives
Frame Rate	30 frames per second