.CAMERA. VI Basic Operations
2 Photographing Images
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Features of tones
There are seven kinds of tones. Choose an easy-to-observe tone according to the subject.
TONE1: Wide dynamic range
Use this tone when gradation from darks to brights is required (as for subjects that have
highlight reflections).
Recommended example: IC chips, part-mounted circuit board, gears, etc.
TONE2: Weak contrast
Use this tone when you want to weaken the contrast.
Recommended example: Cells and tissue, general subjects
TONE3: Standard contrast
Use this tone for ordinary photographing.
Recommended example: Cells and tissue, general subjects
TONE4: Strong contrast
Use this tone when you want to enhance the contrast. The image will have clearly
distinguishable darks and brights.
Recommended example: Cells and tissue, general subjects
TONE5: Linear
There is a linear output proportional to the input from the camera. Use this tone when you
want to examine the difference in luminous energy from photographed data.
Recommended example: DIC observation, PH observation, etc.
TONE6: Provided for metal tissue
Use this tone when you want to suppress unevenness in bright parts while photographing
a high-contrast image.
Recommended example: Metal tissue or ceramics and other materials
TONE7: Enhanced contrast
Use this tone when you want to enhance the contrast during observation.
Recommended example: Metal tissue or ceramics and other materials, photographing
documents with a lens, etc.
(5) Adjusting the white balance
Before photographing an image you need to adjust the white balance of the camera using a
subject that has even whites.
1) When using diascopic illumination for microphotography, photograph a transparent part of
the preparation. When using episcopic illumination for microphotograph or when using a
lens, adjust the white balance with a white subject.
For fluorescent photography, we recommend that the white balance be adjusted under
normal light conditions before photographing an image.
2) Next, adjust the exposure.
It would be ideal if the amount of incident light can be adjusted in such a way that the
shutter speed falls within a range of about 1/7.5 to 1/250 seconds. To adjust the amount
of incident light, adjust the luminous energy of the light source or the iris diaphragm or
use an ND filter.
3) Press WB while in this state, and the white balance will be automatically adjusted.
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