Nikon Eclipse LV100DA-U - Page 74

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(1)
(2) (3)
A
A
DAPI
FITC
TRITC
Texas-Red
A
B
B
B
100%
0%
350 400 450 500 550 600
650 700
Transmittance
Wavelength
: Effective diameter
on the aperture
diaphragm surface
50%
Details on the excitation light balancer
The transmittance for the FITC is designed to keep approximately 100%, because the FITC is
usually dark fluorescent image.
Optical path position
(1)
Between (1) and (2)
(2)
Between (2) and (3)
(3)
TRITC / Texas-Red
0%
Variable (0% to 50%)
50%
Variable (50% to 100%)
100%
DAPI
100%
Variable (100% to 50%)
50%
Variable (50% to 0%)
0%
FITC
100%
100%
100%
100%
100%
III. Operation of Each Part