Chapter 2 Microscopy
7
10
After observation, turn off the light source.
• After observation, turn off the light source and the lamp.
• If the microscope or the light source is to be covered by a dust cover, cool down the light source
sufficiently.
Bright-field microscopy under epi-illumination
2
Set the specimen.
6
Place an objective into the optical path.
7
Episcopic illumination:
Adjust the aperture diaphragm.
9
Observe the specimen
under a magnification
adjusted by zoom operation.
Turn on the light source
and adjust the brightness.
4
Adjust the interpupillary distance
of the binocular part.
5
Adjust the diopters of the eyepieces.
1
Set the optical path selector
to the binocular part position.
8
Focus on the specimen.
8
Adjust the position
of the specimen and then
focus on the specimen.
3
10
After observation,
turn off the light source.
Diascopic illumination:
Adjust the condenser, the aperture
diaphragm, and the field diaphragm.
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