5
SUMMARY OF BRIGHTFIELD OBSERVATION
PROCEDURE
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· Turn the revolving nosepiece to
engage the 10X objective.
#Make sure that the revolving
nosepiece stops with an audible
click.
· Place a specimen on the stage.
(Page 10)
· Turn the X-axis knob and Y-axis
knob to move the specimen into
the light path. (Page 11)
· Set the main switch to “ I ” (ON)
and adjust the brightness with the
light intensity knob. (Page 8)
· Turn the coarse and fine
adjustment knobs to bring the
specimen into focus.
· Adjust the interpupillary distance.
(Page 11)
· When the tilting observation tube is
used, adjust the angle of tilt.
(Page 11)
· Adjust the diopter. (Page 11) · Center the field iris diaphragm.
(Page 13)
· Adjust the aperture iris diaphragm
and field iris diaphragm. (Page 8)
10 Engage the objective to be used for observation in the light path, then readjust the focus.
11 Place the required filter on the filter holder.
12 Re-adjust the aperture iris diaphragm, field iris diaphragm and brightness and start observation.