Olympus CX43 - Transmitted Phase Contrast Observation

25
CX43
1
Centering the ring slit for phase contrast
Engage the 10X objective for phase contrast in the light path and bring
the specimen into focus.
Rotate the turret of the condenser to select Ph1.
Select the condenser turret position (Ph1/Ph2/Ph3) same as the
Ph display
a
of the objective.
Remove the eyepieces and replace it with the centering telescope CT-5.
Rotate the Y-axis knob
b
in the arrow direction to move the stage
to the foremost.
While looking through the centering telescope, rotate the focus ring
at the upper area of the centering telescope to bring the specimen
into focus so that the ring slit (bright ring)
c
in the field of view and
the phase plate (dark ring)
d
of the objective can be viewed clearly.
If the ring slit (bright ring) in the field of view and the phase plate
(dark ring) of the objective cannot be viewed clearly, remove the
specimen from the stage.
Push the centering knob (2 positions) into the condenser turret toward
inside (front side).
Rotate the centering knob (2 positions) keeping them pushed in to
adjust so that the ring slit (bright ring)
c
overlaps the phase plate
(dark ring)
d
.
In a same manner, perform the centering of Ph2 for the 40X objective
for phase contrast and Ph3 for 100X objective for phase contrast.
The same Ph1 is used for the 20X objective for phase contrast
and the 10X objective for phase contrast. The centering for the
20X objective for phase contrast is not necessary.
Remove the centering telescope CT-5 and return the eyepieces to
the tube.
Depending on the specimen status, the centering adjustment
of the ring slit may be necessary by each specimen.
1
2
TIP
3
4
5
TIP
6
7
8
TIP
9
TIP
d
c

×

4
0
/
5
0
.
6
P
h
a
b
5 7