Acquiring and saving images
20
Define calibration
Click this button to determine the calibration data required for the X/Y calibration
of your images. Such a calibration is only necessary and reasonable if you use
your camera with a microscope or with a macro stand with one or more definite
magnification ranges.
The image acquisition software enables you to calibrate images after acquisition.
Calibration means that for each magnification the width and height of an object
area is assigned to the pixel representing that object area. This might be, for
example, 0,63 µm x 0,63 µm for a magnification of 5x. This calibration data var-
ies for each magnification.
1) Place a stage micrometer under your microscope and focus.
The illustration shows a
light microscope's
micrometer.
2) Click the Acquire Image button to acquire an image of the stage microme-
ter.
" The image will be put into the active image buffer.
3) Click the Define calibration button to open the dialog box required to deter-
mine the calibration data.
4) Click the Calibrate... button.
" The Calibrate Image dialog box opens.
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