15
2-4 Darkfield Observation (DF)
Applicable objective power
* With the superwide-field observation (FN 26.5), flare may be observed in the peripheral areas of the field
of view. However, this does not pose problem in photomicrography.
** Objectives with N.A. of no more than 0.7 can be used. (An objective which is equipped with an iris
diaphragm and which can reduce the N.A. to 0.7 or less can also be used.)
}When a transmitted light DIC slider is used in combination, pull out the slider until it clicks in place to
disengage the slider from the light path.
}When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in place
to disengage the analyzer from the light path.
1. Rotate the turret to select the DFA darkfield observation light path.
2. Pull out the polarizer handling knob to disengage the polarizer from the light path.
3. Mount the objective to be used in the revolving nosepiece and rotate the nosepiece to swing the objec-
tive in place.
4. Open the aperture iris diaphragm.
5. Place the specimen on the stage and move the stage up and down to bring the specimen into focus.
6. Remove an eyepiece from the eyepiece sleeve, and look at the objective pupil. Center the darkfield ring
using the optical element centering screw.
7. Insert the eyepiece into the eyepiece sleeve and look at the darkfield image. Repeat centering until the
optimal darkfield effect is obtained.
Power 10X 20X 40X 60X 100X
Darkfield (DF) ¦* ¦** ¦** ¦** ¦**
Top lens (U-TLD) IN (U-TLO cannot be used.)
ax7353_inst.pmd 20.04.2005, 12:2715
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