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Olympus U-UCD8 - Phase Contrast Observation (PH)

Olympus U-UCD8
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U-UCD8
8
Applicable objective power
Power 10X 20X 40X 60X 100X
Phase Contrast (PH) ¦* ¦ ¦ ¦ ¦
Top lens (U-TLD) IN (U-TLO cannot be used.)
* With the superwide-field observation (FN 26.5), flare may be observed in the peripheral areas of the field
of view. However, this does not pose problem in photomicrography.
#When a transmitted light DIC slider (U-DICT, U-DICTS, etc.) is used in combination, pull out the
slider until it clicks in place to disengage the slider from the light path.
#When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in
place to disengage the analyzer from the light path.
1. Rotate the turret to engage the phase contrast ring (U-PH1S, U-PH2S, U-PH3S or U-PH3) that matches the
objective in use.
2. Pull out the polarizer handling knob to disengage the polarizer from the light path.
2-2 Phase Contrast Observation (PH)
· As microscopic specimens are usually low in contrast, reduc-
ing the diaphragm opening to 70% or 80% of the objective’s
N.A. will generally provide an image of acceptable quality. To
check the opening, after completing focus adjustment, remove
one of the eyepieces and look into the empty eyepiece sleeve.
As you stop down the aperture iris diaphragm, the iris dia-
phragm image can be seen in the objective pupil. (Fig. 1)
ax7353_inst.pmd 20.04.2005, 12:278

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