U-UCD8
6
VARIOUS MICROSCOPY PROCEDURES
}If the top lens and optical elements have not been assembled yet, first read Chapter 5, “ASSEMBLY”
(pages 21 to 30).
Applicable objective power
* The NA may slightly be insufficient when a dry type top lens is used. However, this does not pose
problem in normal observations.
#When a transmitted light DIC slider (U-DICT, U-DICTS, etc.) is used in combination, pull out the
slider until it clicks in place to disengage the slider from the light path.
#When a reflected light analyzer (U-AN) is used in combination, pull out the analyzer until it clicks in
place to disengage the analyzer from the light path.
1. Rotate the turret to select the BF brightfield observation light path (no optical element engaged).
2. Pull out the polarizer handling knob to disengage the polarizer from the light path.
3. Mount the objective to be used in the revolving nosepiece and rotate the nosepiece to swing the objec-
tive in place.
4. When using a 2X to 4X objective, swing out the condenser’s top lens. Also open the aperture iris dia-
phragm.
#When the top lens is swung out, the microscope’s field iris diaphragm function as aperture iris
diaphragm.
5. Place the specimen on the stage.
6. Move the stage up and down to bring the specimen into focus.
2-1 Brightfield Observation (BF)
Power 1.25X 2X 4X 10X 20X 40X 60X 100X
Brightfield (BF) – ¦ ¦ ¦ ¦ ¦ ¦* ¦*
Top lens OUT Top lens IN
ax7353_inst.pmd 20.04.2005, 12:276
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