PerkinElmer LAMBDA 265 - Page 25

09931228D
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2. The following dialog box will be displayed.
3. Setup test parameters as follows:
a. Edit Heating(Cooling) Rate (°C/min): Set the heating (cooling) temperature rate in each
temperature range. Sample is measured whenever it reaches the set temperature by the
Rate (°C/min), and the sample is also measured at the Start Temperature and the End
Temperature. Click on Insert and Delete to edit the temperature range and the rate.
- Start Temperature (°C): Enter the start temperature for the measurement.
- End Temperature (°C): Enter the end temperature for the measurement.
- Rate (°C/min): Enter the heating (cooling) rate.
b. Holding Time (min): Enter the holding time before sample measurement after it reaches
the each set temperature.
c. Volume Correction: If volume correction is selected, the baseline corrected absorbance
value is corrected for the thermal expansion of an aqueous buffer. The default equation for
volume correction is:
Volume (T) = 0.99829+104.5 * 10
-6
T+3.5 * 10
-6
T
2