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PerkinElmer LabChip GX User Manual

PerkinElmer LabChip GX
346 pages
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Copyright 2008-2014, PerkinElmer Health Sciences, Inc. All rights reserved.
V4.2
User Manual
LabChip GX/GX II

Table of Contents

Questions and Answers:

PerkinElmer LabChip GX Specifications

General IconGeneral
BrandPerkinElmer
ModelLabChip GX
CategoryMeasuring Instruments
LanguageEnglish

Summary

Preface

Copyright

Details copyright ownership and reproduction rights for the manual.

Trademarks

Lists registered trademarks of PerkinElmer and other companies mentioned.

Content

Information on publication errors, warranty disclaimers, and revision rights.

Proper Equipment Operation

Essential warnings and instructions for safe and correct operation of the instrument.

Contact Us

Contact information for PerkinElmer Technical Support for assistance.

Table of Symbols

Important Symbols

Explanation of symbols used in the manual to denote hazards and important information.

Instrument Safety

Required Training

Ensure personnel are trained in lab and instrument safety practices before operation.

Chemical Safety

Guidelines for safe handling and disposal of chemicals used with the instrument.

Laser Safety

Precautions and warnings regarding the Class 3B laser diodes in the instrument.

Electrical Safety

Information on power supply, voltage, and safe handling of electrical components.

Mechanical Safety

Warnings about pinch hazards from the robotic arm and safe operation practices.

Introduction

Principles of Operation

Explanation of the core gel electrophoresis principles adapted for the LabChip GX chip format.

Software Modes Available

Description of the three operational modes: Instrument, Simulation, and Reviewer.

Operation

Running an Assay

Step-by-step guide to start and execute an assay on the LabChip GX instrument.

Monitoring the Run

How to observe assay progress and data acquisition status during operation.

Saving Data Files

Procedures for saving raw and analyzed data files generated during assays.

Data Analysis

How the Software Analyzes DNA Data

Explanation of how the software processes and analyzes DNA sample data.

Modifying Analysis Parameters

Procedures for adjusting software settings to optimize data evaluation and analysis.

Viewing Analysis Errors and Warnings

How to identify and interpret analysis errors and warnings displayed in the software.

Software Security

Managing User Accounts

Procedures for creating, editing, and managing user accounts and access levels.

Audit Trail

Information on how the software logs all user and system events for security and compliance.

Software Reference

LabChip GX Main Window

Description of the primary software interface, its layout, and components.

Assay Analysis Window

Details on the window for setting and modifying assay parameters and analysis settings.

Graph View

Visual representation of data from each well as an electropherogram.

LabChip GX Instrument Description

Front Panel

Explanation of the controls, buttons, and status indicators on the instrument's front panel.

Specifications

Technical specifications including size, weight, power, and environmental operating conditions.

Maintenance and Service

Cleaning the Chip Cartridge

Procedures for daily and monthly cleaning of the chip cartridge and O-rings.

Troubleshooting and Diagnostics

Error Messages

Overview of common error messages and their potential causes and solutions.

Running the Diagnostics Tests

Instructions for performing diagnostic tests to verify instrument functionality.

Tips and Shortcuts

Single Click

Basic mouse actions for selecting or deselecting wells and navigating views.

Ctrl + Click

Using Ctrl+Click to overlay data from multiple wells for comparison in Graph View.

Right-Click

Accessing context-sensitive shortcut menus for various actions within the software.

Glossary of Terms

Baseline

Defines how a baseline is established for peaks and the algorithms used for subtraction.

Ladder

Explains the use of ladders for aligning data and calculating peak sizes.

Optical Calibration

Explains the process of calibrating instrument optics for consistent fluorescence measurements.

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