Introduction 21
V4.2 LabChip GX User Manual PerkinElmer
Principles of Operation (Continued)
For DNA, RNA, Protein, and Glycan assays, quantitating the
concentration and accurately sizing each fragment are achieved by
comparing against a sizing ladder and running internal standards or
“markers” with each sample. Internal standards of known
concentration are mixed with the sample to aid in quantitation.
The amount of sample sipped into the chip depends on pH, salt
concentration, and buffer additives. The internal standards
normalize these factors so that the software can use the ratio of the
area of the curve of the standard to the unknown peak to determine
concentration directly. The internal standards lie slightly outside the
assay range so they do not interfere with analysis.
Capillary Zone Electrophoresis (CZE) is an electrophoretic
separation technique used to evaluate the charge heterogeneity of
proteins in a sample. For Protein Charge Variant assays, analytes
are separated based on their net charges, with molecules with a
higher net charge migrating faster than those with a lower net
charge. The data is analyzed to calculate the % Relative Amount of
the peaks.
Software Modes Available
The LabChip GX software has three modes available: Instrument,
Simulation, and Reviewer. The mode can be selected when the
LabChip GX software is installed.
Instrument Mode is used to control the LabChip GX instrument
and to review data generated by the LabChip GX.
• The 21 CFR Part 11 Compliance option is a purchased option
that can be used when the software is installed in Instrument
mode.
Simulation Mode is used to simulate running an instrument and to
review data generated by the LabChip GX.
Reviewer Mode is used to review data and develop assays, but
does not control an instrument or display the instrument controls.
If the LabChip GX software is installed in Instrument mode,
shortcuts can be created on the desktop to run the LabChip GX
software in Simulation or Reviewer mode (see “Creating Desktop
Shortcuts” on page 23).
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