Data Analysis 67
V4.2 LabChip GX User Manual PerkinElmer
Using Expected Fragments/ Expected Proteins/
Expected Glycans
You can track expected DNA fragments (EFs), proteins (EPs),
Glycans (EGs), or peaks (EPs) for the samples in a DNA, gDNA,
Protein, Glycan, or Protein Charge Variant assay, respectively.
(Protein, Glycan, and Protein Charge Variant assays are only
supported on LabChip GX II instruments.) You enter the EFs, EPs,
and EGs in the Assay Analysis Window.
Entering EFs, EPs, or EGs in the Assay Analysis Window
1 Select Analysis Analysis Settings on the Menu Bar. The
Assay Analysis Window opens.
2 Click on the Expected Fragments/Proteins/Glycans Tab.
3 Click in the bottom (empty) row in the table.
4 In the Size column, type the size of the expected fragment (bp
for DNA or kb for gDNA), protein (kDa), or glycan (CGU).
5 For CZE assays, type the time of the expected peak, in
seconds, in the Time Column.
6 In the Window (%) column, if desired, change the tolerance
value to allow for variations in the expected fragment/protein/
glycan size or peak time. This value is specified as a percent of
the expected size for that fragment/protein/glycan or time for a
peak. The default is 10% for DNA, protein, and CZE, and 2.5%
for Glycan.
Note: If there are multiple peaks in the tolerance range, the
largest peak is labeled as the expected peak, even if it is not the
exact size specified. To change the peak identified as an
expected peak, see “Forcing Expected Peaks” on page 69.
7 A default color is automatically assigned. To change the color,
click on the color block in the Color column and select the
desired color in the Color window.
8 If desired, change the name shown in the Name column.
9 If desired, change the Property Displayed in the Well Table.
This setting specifies the property that will be displayed in the
Expected Peak column for each expected peak listed in the Well
Table View.
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