PerkinElmer LabChip GX - Table of Contents

Table of Contents 12
V4.2 LabChip GX User Manual PerkinElmer
Table of Contents
Preface ....................................................................................................................2
Instrument Safety ...................................................................................................7
Required Training................................................................................................ 7
Chemical Safety ..................................................................................................8
Laser Safety........................................................................................................ 9
Electrical Safety ................................................................................................ 10
Power Cord Selection .................................................................................. 10
Fuses .......................................................................................................... 11
Mechanical Safety............................................................................................. 11
Introduction...........................................................................................................18
Principles of Operation ...................................................................................... 19
Software Modes Available ................................................................................. 21
Operation ..............................................................................................................22
Opening the LabChip GX Software.................................................................... 23
Creating Desktop Shortcuts............................................................................... 23
Creating a New Assay ....................................................................................... 24
Running an Assay ............................................................................................. 25
Select the Auto Export Settings ......................................................................... 29
Monitoring the Run ............................................................................................ 30
Stopping a Run ................................................................................................. 31
Continuing a Stopped Run ................................................................................ 32
Saving Data Files .............................................................................................. 33
Saving Workspace Files .................................................................................... 34
Selecting Wells using a Sample Names File...................................................... 35
Using a Barcode to Specify the Sample Names File .................................... 36
Adding a New Plate........................................................................................... 38
Placing the Barcode on the Plate ...................................................................... 40
Calibrating the Optics........................................................................................ 41
Data Analysis ........................................................................................................42
How the Software Analyzes DNA Data .............................................................. 43
How the Software Analyzes Protein Data .......................................................... 45
How the Software Analyzes RNA Data .............................................................. 49
How the Software Analyzes Glycan Data........................................................... 54
How the Software Analyzes Protein Charge Variant Data.................................. 57
How the Software Analyzes Genomic DNA Data ............................................... 58
Organizing, Retrieving, and Backing Up Data Files ........................................... 59
Opening a New Workspace ............................................................................... 59
Opening a Data File .......................................................................................... 60
Adding a Collection to a Workspace .................................................................. 61
Selecting the Wells in a Collection .................................................................... 62
Using Sample Names Files ............................................................................... 63
Create a Sample Name File......................................................................... 63