Operation 30
V4.2 LabChip GX User Manual PerkinElmer
Monitoring the Run
The following occurs after a run is started in the LabChip GX
software:
1 The priming and warming steps are performed.
• The priming step fills the channels of the chip with reagent.
(Only performed if the chip holder has been opened since
the last run.)
• The warming step allows the heater plate located in the chip
holder to regulate chip temperature to 30°C.
• Data collection begins after the warming and priming steps
are completed.
2 After the run begins, the Start button on the LabChip GX main
window changes to Stop.
3 The Active Data Tab displays the electropherogram of the well
currently being read. Data is saved to a file with the name
shown above the plate diagram after each well is completed.
4 To view the results for individual wells as data is acquired or
after the run is finished, click a well in the Plate View or Plate
List, a sample name in the Well Table View, or a lane in the Gel
View. Data from the selected well displays in the Overlay
Electropherograms Tab and the Peak Table View.
For more information on data analysis, see “Data Analysis” on
page 42.
5 To stop the run before it is complete, see Stopping a Run.
6 When the assay is complete, Run Successful displays in the
Status line.
7 If desired, remove the plate and/or remove the chip.
To save the analyzed data:
1 Select File Save Workspace. The Save Workspace As
Window opens with the default workspace name as the name of
the data file.
2 If desired, change the location and/or the name of the
workspace file and click the Save button. (Workspace files have
a .gxw file extension.)
To view or re-analyze the data, see Data Analysis.
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