PerkinElmer LabChip GX User Manual

Data Analysis 57
V4.2 LabChip GX User Manual PerkinElmer
How the Software Analyzes Protein Charge
Variant Data
Protein Charge Variant assays are only supported on LabChip GX II
instruments.
The LabChip GX Protein Charge Variant Assay Analysis consists of
the following steps:
1 Raw data is read and stored by the system for each individual
well.
2 The data is filtered (see “Data Filtering” on page 307) and the
resulting electropherograms of all wells are plotted.
3 A baseline is generated for the data using the settings on the
Peak Find tab on the Assay Analysis Window. This baseline is
displayed on the electropherogram when Show Peak Baselines
is selected in the Graph View Properties (see page 164).
4 Peaks are identified (see “Understanding Peak Finding” on
page 108) for all wells and are tabulated by migration time. The
settings of the peak find algorithm can be changed and the data
can be reanalyzed after the run has finished. (See “Reanalyzing
a Data File” on page 95.) The peak find settings can be changed
for all or only certain wells.
5 The % Relative Amount for each peak is calculated as a percent
of total area.

PerkinElmer LabChip GX Specifications

General

Brand	PerkinElmer
Model	LabChip GX
Category	Measuring Instruments
Language	English