Data Analysis 56
V4.2 LabChip GX User Manual PerkinElmer
How the Software Analyzes Glycan Data
(Continued)
7 The standard curve and the markers are used to calculate
glycan sizes in each well from the migration times measured.
8 The area under each peak is calculated.
9 All the sample peak areas are added together and an area ratio
is calculated for the %Area for each peak.
NOTES
• In Glycan assays, the lower marker does not precede all ladder
peaks but appears between the 6th and 7th ladder peak. The
concentration of the lower marker has been made sufficiently
large to avoid confusion with ladder peaks. Since peaks before
the lower marker are ignored, the ladder peaks used for the
sizing ruler range from 7 to 13 CGU.
• The software allows you to define the lower marker. Changing
the selection of the lower marker will lead to quantitative
changes in the calibration, and therefore in the entire data
evaluation.
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