Data Analysis 54
V4.2 LabChip GX User Manual PerkinElmer
How the Software Analyzes Glycan Data
Glycan assays are only supported on LabChip GX II instruments.
The LabChip GX Glycan Assay Analysis consists of the following
steps:
1 Raw data is read and stored by the system for each individual
well.
2 The data is filtered (see “Data Filtering” on page 307) and the
resulting electropherograms of all wells are plotted.
3 Peaks are identified (see “Understanding Peak Finding” on
page 108) for all wells and are tabulated by migration time. The
settings of the peak find algorithm can be changed and the data
can be reanalyzed after the run has finished. (See “Reanalyzing
a Data File” on page 95.) The peak find settings can be changed
for all or only certain wells.
4 A sizing ladder (see Figure 19), which consists of multiples of a
basic sugar molecule, is run from the ladder vial before and after
every 12 samples. The simplest molecule is assigned a size unit
of 1 CGU (Caliper Glucose Unit). The sequence of ladder peaks
is assigned integer size values, providing a migration time to
size ruler for sizing other sugar molecules.
Figure 19. Ladder Graph - Glycan
5 The ladder is analyzed (see “Ladder Analysis” on page 311) and
a standard curve of migration time versus mobility is plotted
from the ladder by interpolation between the individual
glycan/migration points. The standard curve derived from the
data of the ladder well should resemble Figure 20.
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