Glossary of Terms 307
V4.2 LabChip GX User Manual PerkinElmer
Data Files
While running an assay, the raw data received from the instrument
is automatically saved to the plate data file (*.gxd). As the data from
each single well is received, it is saved to the data file. The name of
the data file is specified during the run in the Start Run Window. If a
run is stopped before the run is complete, the data file contains the
data for the completed wells.
After a run is complete, the data is analyzed using the analysis
settings in the assay. The analysis settings are saved in the data file
(.gxd).
After the run, you can change the analysis settings using the Assay
Analysis Window. When the new settings are applied, the data is
reanalyzed and the updated results are displayed. Changes to the
analysis settings are not saved until the workspace is saved. To
save analysis settings without saving the workspace, select
Workspace Save Plate or right-click on the plate name above
the plate diagram on the left side of the window and choose Save
Plate.
Changes to the analysis settings are saved at the end of the plate
data file without overwriting previous settings in the file. The
Restore Plate option can be used to return to previous analysis
settings.
Data Points
Data points are determined by the data collection rate set in the
assay properties.
Show Data Points is an option in the Graph View Properties that
displays the data points used to generate the graph.
Data Filtering
Before identification of peaks in the fluorescence data can proceed,
the raw data must be smoothed to prevent the detection of signal
noise as peaks. For details on the data filtering process, see “Data
Smoothing” on page 108.
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