Data Analysis 79
V4.2 LabChip GX User Manual PerkinElmer
Using the Default Ladder for Alignment
For DNA, gDNA, and RNA assays, after the default ladder is
selected for a data file, the default ladder must be assigned to the
desired wells in the plate.
1 With the plate data file open, select Analysis ïƒ Analysis
Settings to open the Assay Analysis Window, and then click the
Alignment Tab.
2 Select the Align Well Groups to Specified Ladder option.
3 For each group of wells under Align Well Group, select Default
Ladder in the To Ladder column.
4 Click the Apply button to apply the changes to the active data
file or click the Apply Global button to apply the changes to all
data files in the current collection.
Exporting the Default Ladder in an Assay
The default ladder in a data file is included in the analysis settings
for a new assay when the settings are exported as an assay.
Exporting the assay enables you to use the default ladder in future
runs.
1 After clicking the Apply or Apply Global button in the Assay
Analysis Window, the Export as Assay button displays.
2 Click the Export as Assay button. The Export Assay Settings
from Plate to Assay File window opens.
3 Select the desired location for the assay file, type the desired
name for the assay file, and click the Save button.
4 Select the new assay in the Run Tab on the Start Run Window.
The new data file will include the default ladder.
Clearing the Default Ladder in a Plate
To delete the default ladder for a plate and return the alignment to
the ladder settings for the assay type:
1 With the plate data file open, select Analysis ïƒ Analysis
Settings to open the Assay Analysis Window, and then click the
Analysis Tab.
2 Click the Clear Default Ladder button at the bottom of the
Default Ladder table.