Introduction 20
V4.2 LabChip GX User Manual PerkinElmer
Principles of Operation (Continued)
In the chip, each sample is sipped from the microplate by negative
pressure until a sufficient quantity is loaded in the chip. The sample
is then moved electrophoretically into the central channel. As the
fragments move down the central channel, they separate by size,
and then pass the laser, which excites the fluorescent dye bound to
the molecule. The LabChip GX software plots fluorescence intensity
versus time and produces electropherograms for each sample (see
Figure 2).
Figure 2. Electropherogram
The data can be viewed in a gel-like format on the Gel Tab to
achieve the appearance of a slab gel. (The colors of the gel can be
changed.)
Figure 3. Gel View
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