Glossary of Terms 310
V4.2 LabChip GX User Manual PerkinElmer
Inflection Threshold
Peaks that are very close together are identified as a single peak if
the peaks do not have a clear valley between them. The Inflection
Threshold property splits peaks based on the slope. The inflection
threshold defines the value that the slope minimum must be below
to trigger a splitting of the peak. As the threshold is increased, more
peak splitting occurs. For details, see “Understanding Peak Finding”
on page 108.
Lab-on-a-Chip
The generic term for a microfluidic product, signifying a chemical
process or material movement taking place on a microchip. In
contrast to analysis in a standard laboratory that relies on human
intervention at several stages to manipulate or observe samples
and record results, the self-contained lab-on-a-chip represents an
almost hands-free technology.
Ladder
Ladders are used to align the data and calculate the size of data
peaks. (Protein Charge Variant assays do not use ladders or
markers.) The Ladder vial is located in the front right corner of the
microplate carrier, next to the buffer vial. The Ladder is sipped
before the first well and then after every 12 wells. The ladder is
analyzed before sample analysis starts.
The peak sizes and markers defined for the ladder in the assay are
assigned consecutively, starting with the first peak detected in the
ladder. If too few peaks are detected in the ladder well, analysis
stops and an error is generated. Peaks appearing above the upper
marker do not have to be detected. The Peak Table View for the
ladder well shows the peak size and concentration set in the Assay
Analysis Window.
Figure 142. Ladder Graph
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