Glossary of Terms 315
V4.2 LabChip GX User Manual PerkinElmer
Molecular Separation Techniques
Processes such as gel electrophoresis, liquid chromatography, and
capillary electrophoresis, which can separate biomolecular organic
substances from other compounds.
Optical Calibration
The LabChip GX instrument reads fluorescence of a laser
illuminated dye attached to compounds present in a sample. The
absolute value of this fluorescence has little meaning as all
computed quantizations, such as concentration, are derived from
ratios of fluorescence to known standards found in the supplied
ladder. Due to component variations in the optical subsystem, the
same sample delivers different absolute fluorescence on different
instruments, making it somewhat disconcerting to the user when
comparing the e-grams of similar samples run on different
instruments. In order to provide a more consistent absolute
fluorescence for visual comparisons, an optical calibration process
is included in the LabChip GX software.
The Optical Calibration uses a calibrated test chip (Test Chip C),
which has a stable, dry fluorescent material embedded in the
channel. Test Chip C is provided with all new instruments. Test Chip
C is factory-calibrated with a reference contrast value written in the
chip RF tag. Each instrument uses this test chip to determine an
optical gain correction factor, which will scale its optical response to
a common standard.
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