Glossary of Terms 316
V4.2 LabChip GX User Manual PerkinElmer
Peak Baseline
A local peak baseline is calculated for each peak. For isolated
peaks, the local peak baseline is a straight line connecting the start
point with the end point. For peaks that are very close together, an
average baseline is used when the value between the peaks does
not drop to the actual baseline. The peak baseline for each peak in
Figure 144 is shown in a different color.
Figure 144. Peak Baselines
The peak baseline algorithm starts at the earliest peak and checks
whether the end point is within a certain distance from the start of
the next peak. When a cluster of peaks is detected, the peak
baseline is the line joining the first peak's start to the last peak's
end. The start and end points of adjacent peaks in the cluster are
averaged to the same point so that no gaps exist between peaks.
The peak baseline Start Point and End Point can be moved in the
Overlay Electropherograms Tab if Show Peak Baselines is
selected in the Graph View Properties.
Peak Height
The value at the apex of the peak minus the local baseline start
value.