Main Applications
Setting up a Run for Purification of Genomic DNA from Whole Blood Samples
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Preparing the Samples
To prepare fresh blood samples
Visually check the source plate to ensure that the samples are pipetted into each individual well of the
source plate.
To prepare frozen blood samples
Visually check the source plate to ensure that the samples are pipetted into each individual well of the
source plate.
Blood rims on the walls of the wells might influence the quality of the eluted nucleic acid.
When precipitates have formed, a centrifugation step is required (e.g., 10 min at 1,900 x g for 10 ml
tubes).
Click the Expiration Date cell and enter the date until when the internal control reagent is valid.
Internal controls are valid up to the next day by default.
Click the Tests cell and select the 96 as number of samples for which the internal control is to be ap-
plied.
The Fill Volume field displays the corresponding internal control volume that is needed for the specified
number of samples.
Click OK to save your settings. The Edit Internal Control Barcode dialog box closes.
Click OK to save your settings. The Settings dialog box closes.
Ensure that there are no clots in the anticoagulated whole blood samples.
Pipette 200 µl of anticoagulated whole blood into the individual wells of a processing cartridge. This car-
tridge is then defined as the source plate.
Pipette the samples into the bottom of the wells using aerosol-preventive tips.
Ensure that the source plate is free of blood rims on the walls of the wells.
Ensure that the samples have reached room temperature before starting a purification run.
Take the anticoagulated whole blood samples out of the freezer.
Thaw the samples under slight agitation, e.g, using a laboratory roller.
Ensure that there are no clots in the anticoagulated whole blood samples.
Pipette 200 µl of anticoagulated whole blood into the individual wells of a processing cartridge. This
cartridge is then defined as the source plate.
Pipette the samples into the bottom of the wells using aerosol-preventive tips.
Ensure that the source plate is free of blood rims on the walls of the wells.
Ensure that the samples have reached room temperature before starting a purification run.
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