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Roche MagNA Pure 96 User Manual

Roche MagNA Pure 96
72 pages
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MagNA Pure 96 System
Customer Training Guide, Version 1.0
Software Version 2.0
December 2010
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Roche MagNA Pure 96 Specifications

General IconGeneral
TypeNucleic acid extraction system
Elution Volume50 µl to 200 µl
AutomationFully automated
SoftwareMagNA Pure 96 Software
Sample Capacity96 samples
Sample VolumeUp to 1000 µl
Sample Input Volume200 µL to 1 mL
Processing TimeApproximately 1 hour for 96 samples
TechnologyMagnetic bead technology
ApplicationsMolecular diagnostics, research
CompatibilityCompatible with various sample types including blood, plasma, and tissue
Power Requirements50 – 60 Hz
ThroughputUp to 96 samples in approximately 1 hour

Summary

Prologue and Introduction

Revision History

Details changes to the training guide's versions.

Contact Addresses

Provides contact information for Roche Diagnostics.

Trademarks and Intended Use

Lists product trademarks and specifies the system's intended use and limitations.

Guide Conventions and Symbols

Explains text conventions, abbreviations, and visual symbols used in the guide.

Warnings and Precautions

Handling Precautions

Safety guidelines for operating the instrument and handling biological samples.

Electrical Safety

Safety instructions related to electrical hazards, grounding, and cord maintenance.

System Start-up

Starting the Instrument and Software

Instructions for powering on the instrument and launching the MagNA Pure 96 Software.

Priming the Instrument

Steps to prime the instrument's fluid system before operational use.

System Fluid Management

Procedures for replacing internal and external system fluid containers.

Waste Container Management

Steps for draining internal and external waste containers.

Main Applications

Setting up DNA Purification

Guide for setting up a run for genomic DNA purification from whole blood.

Setting up RNA Purification

Guide for setting up a run for cellular RNA purification from cultured cells.

Performing a Run

Steps to start, monitor, and end a purification run.

Unloading the Instrument

Procedures for removing components and disposables after a run.

Performing a Volume Transfer

Defining Transfer Parameters

Setting software parameters for transferring sample eluates to another plate.

Loading Stage for Transfer

Loading components onto the instrument stage for volume transfer.

Resource Check for Transfer

Verifying components are correctly loaded for volume transfer.

Shutting Down the System

Shutting Down the Software

Steps to properly exit the MagNA Pure 96 software.

Switching Off the Instrument

Procedure for safely powering off the instrument.