Ryf Axio Lab.A1 - Page 95

OPERATION
Axio Lab.A1 Lighting and contrasting method in transmitted light Carl Zeiss
04/2013 430037-7144-001 95
x Limit to aperture to a value of about 0.2.
x Turn the rotary stage Pol until the specimen is almost obliterated, i.e. completely dark.
x Rotate the stage once (by 45°) so that the specimen is in a diagonal position (bright).
The interference intensity or color leads to the following conclusion:
 If more or less strong interference colors appear on the specimen, the path difference ranges
approximately between 1/2 O and 5 O.
The suitable compensator is:
tilting compensator B 0-5
O
O
.
 If the specimen-side color changes from light gray/white to a strong interference color, when a
compensator O (473704-0000-000) is inserted in the compensator slot, the path difference is
(1/4 ...1/2) O.
A prerequisite for occurrence of the color change effect may be the evaluation in two specimen
positions rotated at an angle of 90° from one another, plus a centered stage.
The suitable compensator is:
tilting compensator B 0-5
O
or the compensation method according to DE SENARMONT up to 1 O
using the Senarmont compensator 546/4 nm.
The compensation method according to DE SENARMONT requires the use of the rotatable
analyzer.
 After insertion of the compensator O and rotation of the specimen by 90°, the interference color
remains white; in this case, however, it is a "higher-order white" and thus the path difference is > 5 O.
The suitable compensator is:
tilting compensator K 0-30
O
(Accessory 000000-1115-698)
 A dark gray as appearing interference color suggests very small path differences (O/10 or 54.6 nm).
The suitable compensator is:
Brace-Köhler rotary compensator
O
/10 (Accessory 000000-1115-703).
x Insert the compensator into the slit as far as it will go.
The accompanying instructions must be observed for measurement preparation and procedure.