Sutter Instrument P-97 - Page 8

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3.3.3 AIR MODE < 3 > ....................................................................................................................18
3.3.4 AIR TIME AT START OF PULL < 4 >...............................................................................19
3.3.5 AIR TIME AT END OF PULL < 5 > ...................................................................................19
3.3.6 RESET TIME AND DATE < 6 >..........................................................................................19
3.3.7 WRITE-PROTECT THE PROGRAM < 7 > .......................................................................19
3.3.8 MEMORY TEST < 8 >...........................................................................................................20
3.4 Pulling Pipettes...............................................................................................................................20
3.4.1 Procedures ................................................................................................................................20
3.4.2 Notes on Program Operation..................................................................................................21
3.5 Parameter Adjustment...................................................................................................................21
3.5.1 General Information ................................................................................................................21
3.5.2 Micropipette/Microinjection Needle Fabrication..................................................................22
3.5.2.1 HEAT..................................................................................................................................22
3.5.2.2 PULL Strength..................................................................................................................22
3.5.2.3 VELOCITY (Trip Point)...................................................................................................22
3.5.2.4 TIME Mode (Cooling) .......................................................................................................23
3.5.2.5 Delay Mode (Cooling)........................................................................................................23
3.5.2.6 PRESSURE Adjustment ..................................................................................................24
3.5.2.7 Filament Width..................................................................................................................24
3.5.3 Patch Pipette Fabrication .......................................................................................................24
3.5.3.1 HEAT..................................................................................................................................24
3.5.3.2 PULL Strength..................................................................................................................25
3.5.3.3 VELOCITY (Trip Point)...................................................................................................25
3.5.3.4 Cooling ................................................................................................................................25
3.5.3.5 PRESSURE........................................................................................................................25
3.5.4 Step-by-Step Patch Pipette Programming ............................................................................25
3.5.5 Technical Tips for Pulling Micropipettes ..............................................................................27
3.5.5.1 Regulating the Time it takes to pull a Sharp Pipette....................................................27
3.5.5.1.1 Pipette Position...........................................................................................................27
3.5.5.2 Regulating the Length and Tip Size of a Sharp Pipette ...............................................27
3.5.5.2.1 Filament Width...........................................................................................................27
3.5.5.2.2 Air Flow .......................................................................................................................27
3.5.5.2.3 DELAY Mode of Active Cooling ................................................................................27
3.5.6 Fire Polishing............................................................................................................................28
3.6 Heating Filaments ..........................................................................................................................29
3.6.1 General Information ................................................................................................................29
3.6.2 Filament Positioning and Aligning ........................................................................................29
3.6.3 Box Filament ............................................................................................................................30
3.6.3.1 Positioning..........................................................................................................................30
3.6.3.2 Geometry ............................................................................................................................31
3.6.4 Trough Filament ......................................................................................................................31
3.6.4.1 Positioning..........................................................................................................................32
3.6.4.2 Geometry ............................................................................................................................32
4. MAINTENANCE...............................................................................................................................33
4.1 Cleaning ...........................................................................................................................................33
4.2 Heating Filament Replacement ....................................................................................................33
4.2.1 Filament Replacement (Step by Step) ...................................................................................33
4.2.2 Air jet Position..........................................................................................................................33
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)