Sutter Instrument P-97 - Page 9

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4.2.3 Positioning the Filament in Relation to the Glass Capillary ..............................................33
4.3 Pulley Adjustment ..........................................................................................................................35
4.4 Regeneration of Drierite Granules................................................................................................36
4.4.1 Removing the Canister ............................................................................................................36
4.4.2 Replacing the Granules ...........................................................................................................36
4.4.3 Reinstalling the Canister.........................................................................................................37
5. TROUBLESHOOTING ....................................................................................................................39
5.1 Controlling Pipette Tip Shapes .....................................................................................................39
5.1.1 Problem: What type of glass should be used? .......................................................................39
5.1.2 Problem: The resistance of pulled pipettes is too low. How can a higher-resistance
pipette be pulled?...............................................................................................................................39
5.1.3 Problem: Okay, but how can tips be made even smaller. ....................................................39
5.1.4 Problem: How can the size of a patch-pipette be increased?...............................................40
5.1.5 Problem: Patch-pipette tips vary in size from pull to pull...................................................40
5.1.6 Problem: Difficulty making an injection pipette with a 0.5 – 1 mm tip that also has a
very short final taper and tip (20 to 50 mm long). How can this be done? .................................40
5.1.7 Problem: The electrodes are bent. How can they be made to pull straight? .....................40
5.1.8 Problem: One electrode is much longer than the other electrode. .....................................40
5.1.9 Problem: The shape and resistance of the pipette changes from pull to pull....................41
5.2 Controller problems........................................................................................................................41
5.2.1 Problem: The filament does not light up when pull is pressed...........................................41
5.2.2 Problem: Display blank, fan not on, or locked up/overlap...................................................42
5.2.3 Problem: Display only shows a cursor. ..................................................................................42
5.2.4 Problem: Displayed program values are not correct ............................................................42
5.3 Technical Support...........................................................................................................................42
APPENDIX A. Limited Warranty........................................................................................................43
APPENDIX B. Accessories....................................................................................................................45
Filaments ...............................................................................................................................................45
Capillary Glass Tubing.........................................................................................................................45
Other Accessories..................................................................................................................................45
APPENDIX C. Fuse Replacement........................................................................................................47
APPENDIX D. Technical Specifications..............................................................................................49
APPENDIX E. Drierite Material Safety Data Sheet ..........................................................................51
INDEX ....................................................................................................................................................53
TABLE OF FIGURES
Figure 1-1. P-97 Front panel. .................................................................................................................... 2
Figure 1-2. Program Display. .................................................................................................................... 3
Figure 1-3. P-97 base plate (top view, cover removed). ..........................................................................5
Figure 1-4. Filament Block Assembly. ..................................................................................................... 6
Figure 1-5. Upper cable pulley assembly. ................................................................................................ 7
Figure 2-1. P-97 Cabinet (rear view), showing power entry module. ................................................. 10
P-97 FLAMING/BROWN MICROPIPETTE PULLER OPERATION MANUAL – REV. 2.43 - DOM (20161118)