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● This adjustment will enable the user to observe the specimen with both
eyes.
3. Diopter adjustment
● Diopter adjustment compensates for the dierences in vision between
the left and right eyes. In addition to making observation through both
eyes easier, this adjustment also reduces the extent to which focusing is
lost when the objective magnication is changed. In particular, this occurs
when a low magnication objective is used.
● The left eyepiece tube has a diopter ring to compensate for slight
dierences in the focusing of each eye.
● Set the diopter to “0” position and turn the nosepiece to 10x agnication.
● Using the right eye only and viewing through the right-hand eyepiece,
adjust the focus with the microscope coarse or ne adjustment until the
image of the specimen is at its sharpest.
● Using the left eye only and viewing through the left-hand eyepiece, turn
the diopter ring to correct focus for left eye until the specimen image is at
its sharpest.
● Change the magnication to higher magnication to verify the result and
if necessary repeat the procedure to match the sharpness for the higher
magnication.
● Keep this diopter position for all magnication/lenses. The diopter
position for each user can be recorded from the scale, so it can be easily
reset.
4. Coarse and ne focusing
● Focusing is carried out with the coarse and ne focus knobs at the left