MINI-V_I_rev0507US Page
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5. USER'S INSTRUCTIONS
5.1. GENERAL RECOMMENDATIONS
The work area
of the
cabinet should
not be
used
as for
storing laboratory equipment. This
could produce
an
accumulation
of
dust which
is
both unnecessary
and
dangerous
for
working
in
sterile conditions.
Even
if the
work area
is
under clean sterile laminar flow, that surrounding
the
cabinet
can
be contaminated.
The
sources
of
contamination should
be
known
so
that they
can be
avoided when inserting arms, material
etc.
All
the
material necessary
for
work must
be
free
of
particles
and
cleaned before
it
enters.
Materials like: paper, wood, cardboard, pencils, erasing rubbers,
etc.,
should
not be
inserted
as they
all
shed large amounts
of
particles.
Both before
and
after work,
it is
advisable
to
wash arms, hands
and
finger nails with
germicide soap. Personnel should avoid touching their mouths
and
eyes.
Long sleeved white coats
are
advised with tight cuffs,
and, for
special work, protecting
gloves,
(or
alternatively, over-sleeves). Both
the
white coats
and the
over-sleeves must
be
made
of
materials least likely
to
shed fibre
and
particles.
The cabinet should
be
started
10
minutes before work begins. This sweeps
the
work area
and material inserted
of
particles.
If pipettes
are
used, they should
be
mechanic suction type; suction
by
mouth must never
be used
as it is
easy
to
inhale aerosols
so
caused.
When platinum handles must
be
used, electric incinerators
or,
better
still,
disposable single-
use handles
are
advised.
If
the
nature
of the
work
to be
done involves using
a gas
flame from
a
Bunsen
(or
similar)
burner,
the
button opening constant pressure type
is
advised.
It
must
be
pointed
out
that
a
constant flame burner creates
a
considerable amount
of
turbulence. Remember that
too
large
a
flame
can
burn
the
absolute filters.
Shadows
and
turbulence produced
by
objects, equipment
and
material
in the
work area,
must
be
taken into account before work begins
to
assess their possible effect.
It
should
be
remembered that
air
flow laminarity
is
only recovered
at a
distance
of up of 2.5
times
the
diameter
of the
object causing
the
obstruction.
If flasks
or
tubes
are
used, these should, preferably,
be
screw
cap
rather than cotton
stopped,
as the
latter sheds large amounts
of
particles.
As soon
as the
work finishes,
all the
disposable products, such
as:
handles, Petri plates,
etc.,
as
well
as
means
of
culture, samples, tubes, flasks
and the
like,
are to be
taken
out of
the cabinet
and
emptied into impermeable bags, which
can be
sterilized
if
necessary.
During work
in the
cabinet
and
cleaning, care must
be
taken
not to
damage
the
HEPA filters
by knocking, squirting liquid
at or
splashing
it.
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