SECTION 2
GETTING STARTED
2.5 Using Optimol Osmolality Standards
25
5
Sample directly from the ampule, using a fresh micropipettor tip
each time to avoid contamination of the solution.
6
Discard any solution that remains after finishing your calibration
procedures.
Optimol standards are packaged in 60-ampule cartons designed for
convenient shipment and storage. See Appendix B for ordering
information.
Assuring Accurate Measurements
The accuracy of reported osmolality is directly linked to the accuracy
of the calibrating standard solutions. While these solutions have
exact specified osmolality at the time they are opened, osmolality
inevitably increases as water evaporates.
Always adhere to the following guidelines when using Optimol
ampule standards:
•
Since the specified value of osmolality is certain only at the time
the ampule is opened, do not rely on any opened ampule if you
are uncertain of how long its contents have been exposed to
evaporation.
•
Sample directly from the ampule–do not transfer standard
solution from ampules to other containers.
•
Always follow the instructions in Section 3.5 to calibrate your
Vapro osmometer, and always verify calibration prior to running
any unknowns.
See Appendix E for more information.
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