SECTION 3
OPERATING THE VAPRO OSMOMETER
3.2 Loading Samples
When first using the Vapro system, practice the loading procedure using
the micropipettor and the 290 mmol/kg standard. Record the value dis-
played at the end of the cycle, when the “In Process” display goes out
and the chime sounds. Practice this procedure until you can obtain
sequential results with a spread of less than 6 mmol/kg. Consistent tim-
ing during loading is important for optimum repeatability. This will come
naturally after a few samples.
NOTE:
While you practice, do not be concerned if the instrument readings do not
agree with the specified concentration of the solution. When you feel comfort-
able with the procedure and are able to obtain repeatable results, calibrate the
instrument using the instructions in Section 3.5, then run the Clean Test
(Section 3.4).
Sample Volume
The optimum sample volume (10 microliters) should fully saturate one
of the SS-033 sample discs. The osmometer accommodates varia-
tions in sample volume as great as ± 10 percent (9 microliters to 11
microliters) without noticeable variation in indicated osmolality.
34
CAUTION!
Samples greater than 11 microliters can contaminate the thermocouple.
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