Initial or Routine Calibration
1
Run a 290 mmol/kg standard.
If the osmometer reads within ± 3 mmol/kg of
the standard (287 to 293), it is within accept-
able calibration limits. In that case, skip to Step
4. If calibration is needed, proceed to Step 2.
2
With the chamber still closed, press SELECT to
reveal the Function Menu. The selector arrow
should be pointing at Calibrate.
3
Press ENTER. The instrument calibrates to the
standard.
4
Repeat this sequence using the 1000 mmol/kg
and 100 mmol/kg standards to establish base-
line calibration for these standards. If the read-
ing is not within ± 5 mmol/kg of the standard
value for the 1000 mmol/kg standard, or ± 2
mmol/kg for the 100 mmol/kg standard, perform
steps 2 and 3.
Calibration Method for Maximum Calibration
Accuracy
When you need maximum accuracy, run the following
calibration sequence.
1
Select Average from the Mode Menu.
2
Run three consecutive assays using 290
mmol/kg standard.
3
Select CALIBRATE and press ENTER.
The instrument calibrates on the average of the
three samples. This method can also be used for
100 and 1000 mmol/kg standards.
SECTION 3
OPERATING THE VAPRO OSMOMETER
3.5 Calibrating
42
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