APPENDIX
D
Special Application Notes
PROCEDURE FOR VERY SMALL SAMPLES
You can measure samples with very low volumes (under 4 µL)
using the following procedures.
Sample discs must be hand made from high-grade filter paper
(Whatman #1 or equivalent) using a high-precision 1/8 inch diame-
ter paper punch to produce discs with a very clean edge.
Required Equipment
•
Wescor low-volume sample holder (AC-063)
•
High quality round hole paper punch, 1/8" diameter (Mieth or
equivalent)
•
High quality 2 µL pipette, which will deliver precisely 2 microliters
or less
•
Pipette tips (short)
•
Tweezers
•
Teasing needle
•
Whatman #1 Filter Paper or equivalent
•
Lint-free tissue paper
•
Cotton-tipped applicators
NOTE:
Maintain a stable ambient temperature. Heat, cold, air currents and temper-
ature fluctuations which vary more than approximately 0.3° C within a 10 to
15 minute time frame, generally will result in poor quality data. You should
monitor the Temp Drift Scale for ambient temperature fluctuations which
will interfere with instrument accuracy.
Technique, including timing, is vitally important to obtaining good
data while conducting very low volume tests.
82
To Next Page
Loading...