APPENDIX
D
Special Application Notes
Instructions
Prior to running the 2 µL samples, calibrate the instrument using 10
µL samples at the 290, 1000, and 100 mmol/kg calibration set
points.
1
Insert the 2 µL sample holder and calibrate on the 290 mmol/kg
standard. If the osmolality of your samples are close to the 290
calibration set point it is not necessary to calibrate at the 1000
mmol/kg level,
2
Load a single paper disc into the center of the special sample
holder. You may need to use the teasing needle and the
tweezers to separate discs that are stuck together.
3
Place sample into the center of paper disc. Be sure to touch
the pipette onto the disc as in regular procedure. Be sure the disc
is completely saturated.
4
Close the sample chamber to begin the measurement cycle.
5
When the measurement is complete, open the sample cham-
ber and retract the sample slide.
6
Thoroughly clean the sample holder of all sample material
using lint-free tissue and a cotton-tipped applicator.
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