Inner Workings – Triple Quadrupole MS versus Single Quadrupole MS 2
How Triggered Dynamic MRM works
Agilent 6400 Series Triple Quad LC/MS Concepts Guide 33
How Triggered Dynamic MRM works
Triggered Dynamic MRM occurs when criteria for primary
MRMs trigger confirmatory (secondary) MRMs to be acquired
for a compound. If the abundances of the Primary MRMs are
higher than the set thresholds and other criteria are met, then
the confirmatory (or secondary) MRMs are acquired. You can
have multiple primary MRMs per compound, and you can
specify up to two of these as Trigger MRMs for each compound.
You can also have multiple secondary transitions for each
compound. All transitions with the same Compound Name
belong to the same compound.
Figure 14 Explanation of threshold for Triggered Dynamic MRM
In Figure 14, only the Trigger MRMs are acquired until the
abundance of each of the Trigger MRMs is higher than the
thresholds you entered. After the abundances for each Trigger
MRM is higher than the threshold, then the secondary
transitions may be acquired, depending on the Trigger
Entrance Delay, Trigger Delay and Trigger Window. These
additional criteria are discussed in the next section.
In the Scan Segments table, you specify which transitions are
Primary transitions by marking the check box in the Primary
column. These transitions are monitored within the peak
retention time window specified for the compound. You also can
specify one or two of these primary transitions as Trigger MRMs
by marking the check box in the Trigger column. Any transition
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