5 Operating in EI Mode
To Perform High-Mass Testing
132 5977B Series MSD Operating Manual
To Perform High-Mass Testing
Materials needed
• PFHT High Mass checkout sample 3 × 1 mL (5188-5357)
Procedure
1 Load the autotune file (atune.u, HES_atune.u, etune.u) then auto tune the
MSD. (See “To Tune the MSD in EI Mode” on page 127.)
2 Resolve the PFHT.M method under
MassHunter\MSD\x\methods\checkout\PFHT.M
where x is the instrument number being used.
3 Update and save the method.
4 Load the PFHT calibration sample into a vial and place in position 2.
5 In Instrument Control view select High Mass Check from the Checkout
menu.
6 Follow the instructions on screen.
7 The run is completed, and results are printed within 5 minutes. (See
Figure 43 on page 133.)
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