Prior to loading the plate, verify that no bubbles are present at the
bottoms of the tubes, beneath the liquid. While it is critical to remove
any bubbles from the bottom of the tubes, small bubbles near the liquid
meniscus should not affect the test results.
Important Bubbles trapped at the bottom of the tubes will interfere
with fluorescence readings, invalidating the test.
2. The first time that the MxPro QPCR software is opened using an
instrument-connected computer, the
Instrumentation Qualification
Test-Start
dialog box will appear. (This dialog box may also be opened
by selecting
Qualification Test from the Instrument menu.) After the
plate is prepared, click
Next.
A series of filter set dialog boxes may appear. Accept the default filter
set changes and settings.
3. The
Instrumentation Qualification Test-Start Run dialog box will
appear, providing an opportunity to enter any comments for the
qualification test.
16 Mx3000P and Mx3005P QPCR Systems Setup and User’s Guide
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