S430 User Manual
56
6.1.3 Triple Wavelengths
A
1
, A
2
and A
3
are the Fluorescence at λ
1
, λ
2
and λ
3
.
2
–
1
2
3
2
3
1
1
3
(
It has to beλ
1
>λ
2
>λ
3
or λ
1
<λ
2
<λ
3
)
6.2 DETAILS ON QUANTITATIVE
Prolab provides 3 calibration types: Linear working curve, Quadratic working curve
and Cubic working curve. All of them are not forced through the 0 coordinates.
6.2.1 Linear Working Curve (1st order)
The calculation formula is as follow:
1
𝐾
0
Where,
C : Concentration of each sample (input value)
A : Abs. of each sample (measured value)
K
1
and K
0
are calculated by the least squares
method.
Suppose there are n data points(
A
n
，C
n
)，then
λ
1
A
1
λ
2
A
2
λ
3
A
A
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